Effect of hypothermic machine perfusion on the metabolism of liver in rats
10.3760/cma.j.issn.1007-8118.2015.09.013
- VernacularTitle:低温机械灌注对大鼠供肝代谢作用的影响
- Author:
Xian LI
;
Wei WANG
;
Xiaoyan HU
;
Yanfeng WANG
;
Yan XIONG
;
Qifa YE
- Publication Type:Journal Article
- Keywords:
Hypothermic machine perfusion;
Liver glycogen content;
Resistance index;
Malondialdehyde;
Donor liver;
Rats
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(9):625-628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the metabolic variations in liver after 30 min warm ischemic injury,and the effect of hypothermic machine perfusion on the metabolism in rats.Methods 40 SD male rats were randomly divided into 4 groups:Group A,the liver underwent warm ischemia for 0 min and cold storage (CS) for5 h; Group B,the liver was treated by warm ischemia for0 min,CS for4 h and then machine perfusion (MP) for 1 h; Group C,the liver suffered from warm ischemia for 30 min and CS for 5 h; and Group D,the liver was treated by warm ischemia for 30 min plus CS for 4 h plus MP for 1 h.During the MP process,the perfusion resistance index was recorded every 10 min,and the liver glycogen content and malondialdehyde (MDA) value were also detected.Results The hepatic glycogen content decreased after MP treatment,but there was no statistical significance (P ＞ 0.05).No differences on MDA contents was found between Group A and B (P ＞ 0.05),while MDA in Group D was significantly higher than that in Group C (P ＜ 0.05).After hypothermic MP treatment,the liver resistance index value was significantly reduced.Conclusion MP could reduce the resistance index but increase metabolic rate in liver undergoing warm ischemic injury,thus producing more lipid peroxides.
