Effects of up and down-regulation of Cox-2 expression on radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice
10.3760/cma.j.issn.0254-5098.2015.10.004
- VernacularTitle:双向调控Cox-2表达对人食管癌EC9706细胞裸鼠移植瘤放射敏感性的影响
- Author:
Dongmei LIU
;
Hui WU
;
Yanhui MA
;
Wenguang ZHANG
- Publication Type:Journal Article
- Keywords:
Esophageal cancer;
EC9706;
Cox-2;
Xenograft tumor;
Nude mice
- From:
Chinese Journal of Radiological Medicine and Protection
2015;35(10):734-737
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of up-and down-regulation of Cox-2 expression on the growth and radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice.Methods Cox-2 specific siRNA and Cox-2 gene eukaryotic expression vector were constructed and transfected to esophageal cancer cells EC9706, and the stable transfected cell lines were obtained by the method of G418 screening.The expressions of Cox-2 mRNA and its protein were detected by RT-PCR and Western blot, respectively.The inhibitory effects of Cox-2 regulation combined with X-ray irradiation on cancer cell growth were detected by the nude mouse xenograft assay.Results Cox-2 gene expression was significantly decreased in the Cox-2 down-regulated group and increased in the Cox-2 up-regulated group.Compared with the control group without gene transfer, the average volumes of EC9706 xenograft tumor in the Cox-2 up-and down-regulated group significantly decreased (F =34.26, P ＜ 0.05) and increased (F =26.38, P ＜ 0.05) , respectively.After 20 Gy X-ray irradiation, the average volume of xenograft was significantly reduced in the Cox-2 down-regulated group (F =16.35, P ＜ 0.05) , but it had no significantly changes in the Cox-2 up-regulated group.Conclusions Down-regulation of Cox-2 expression inhibits the growth of human esophageal cancer EC9706 xenograft in a nude mice and increases cell radiation sensitivity, but upregulation of Cox-2 expression makes tumor cells to become radioresistant.
