Pharmacokinetics of Glutathione and Its Metabolites in Normal Subjects.
10.3346/jkms.2005.20.5.721
- Author:
Sae Yong HONG
1
;
Hyo Wook GIL
;
Jong Oh YANG
;
Eun Young LEE
;
Hyung Kee KIM
;
Soo Hyun KIM
;
Young Ho CHUNG
;
Soo Kyung HWANG
;
Zee Won LEE
Author Information
1. Department of Internal Medicine and Clinical Research Institute, Soonchunhyang University Cheonan Hospital, Cheonan, Korea. eylee@sch.ac.kr
- Publication Type:Original Article ; Clinical Trial ; Research Support, Non-U.S. Gov't
- Keywords:
Cysteine;
Cystine;
Glutathione;
Methionine;
Paraquat;
Reactive Oxygen Species;
Pharmacokinetics
- MeSH:
Adult;
Amino Acids/*blood;
Animals;
Dose-Response Relationship, Drug;
Glutathione/administration and dosage/*blood/*pharmacokinetics;
Humans;
Kinetics;
Male;
Metabolic Clearance Rate/drug effects;
Mice;
Reactive Oxygen Species/*metabolism;
Research Support, Non-U.S. Gov't;
Swiss 3T3 Cells
- From:Journal of Korean Medical Science
2005;20(5):721-726
- CountryRepublic of Korea
- Language:English
-
Abstract:
To determine the loading and maintenance dosage of glutathione (GSH) for patients suffering from reactive oxygen species (ROS) injury such as acute paraquat intoxication, a kinetic study of reduced GSH was performed in synchrony with that of cysteine (Cys), cystine (Cys2), and methionine (Met). Human subject's porticipitation was voluntary. The effective dose of Cys, Cys2, and Met against ROS in fibroblast cells generated by paraquat was assessed using laser scanning confocal microscopy. Both Cys and Met suppressed ROS in a dose-dependent manner at concentrations of 1-1,000 micrometer; the concentration required to suppress ROS by 50% was 10 micrometer for Cys and 50 micrometer for Met. Using metabolite kinetics with the assumption that Cys and Met are the metabolites of GSH, expected concentrations of Cys and Met of above 20 and 50 micrometer were estimated when GSH was administered at 50 mg/kg body weights every 205.4 min for Cys and 427.4 min for Met.
