Antioxidant effect of lidocaine and procaine on reactive oxygen species-induced endothelial dysfunction in the rabbit abdominal aorta.
10.4097/kjae.2010.59.2.104
- Author:
Jae Myeong LEE
1
;
Jung Kook SUH
;
Ji Seon JEONG
;
Sang Yun CHO
;
Dong Won KIM
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, Hanyang University, Seoul, Korea. jksuh@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Catalase;
Endothelium;
Lidocaine;
Procaine;
Reactive oxygen species;
3-amino-1,2,4-triazole
- MeSH:
Acetylcholine;
Amitrole;
Antioxidants;
Aorta;
Aorta, Abdominal;
Baths;
Catalase;
Deferoxamine;
Electrolysis;
Endothelium;
Hydrogen Peroxide;
Lidocaine;
Lipid Peroxidation;
Mannitol;
Oxygen;
Phenylephrine;
Procaine;
Rabbits;
Reactive Oxygen Species;
Relaxation;
Sodium Salicylate;
Vasodilation
- From:Korean Journal of Anesthesiology
2010;59(2):104-110
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Reactive oxygen species (ROS) induce lipid peroxidation and tissue damage in the endothelium. We tested the antioxidant effect of lidocaine and procaine on ROS-induced endothelial damage in the rabbit aorta. METHODS: Aortic rings isolated from rabbits were suspended in an organ bath filled with Krebs-Henseleit (K-H) solution bubbled with 5% CO2 and 95% O2 at 37.5degrees C. After precontraction with phenylephrine (PE, 10(-6) M), changes in tension were recorded following a cumulative administration of acetylcholine (ACh 3 x 10(-8) to 10(-6) M). Differences were measured as percentages of ACh-induced relaxation of aortic rings before and after exposure to ROS as generated by electrolysis of the K-H solution. The aortic rings were pretreated with lidocaine or procaine (10(-5) M to 3 x 10(-3) M) to compare their effects, as well as ROS scavengers, catalase, mannitol, sodium salicylate, and deferoxamine, and a catalase inhibitor, 3-amino-1,2,4-triazole (3AT). RESULTS: Lidocaine and procaine dose-dependently maintained endothelium-dependent relaxation induced by ACh despite ROS activity (P < 0.05 vs control value). The 3AT pretreated procaine (3 x 10(-3) M) group decreased more significantly than the un-pretreated procaine group (P < 0.05). CONCLUSIONS: These findings suggest that lidocaine and procaine dose-dependently preserve endothelium-dependent vasorelaxation against ROS attack, potentially via hydrogen peroxide scavenging.
