The difference and correlation of the expression of hypoxia reaction genes at different time of kainic acidinduced epilepsy in rats
10.3760/cma.j.issn.1674-6554.2015.07.002
- VernacularTitle:红藻氨酸致癫痫大鼠不同时点低氧反应基因表达量的差异及相关性分析
- Author:
Tingxian NIU
;
Xiaoming FENG
;
Xiaohong LUO
;
Xiaoyu GUO
;
Lu LU
;
Hongyi WANG
;
Jian SHEN
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Hypoxia reaction genes;
Correlation;
Real time quantitative PCR
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(7):580-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the difference of the expression of hypoxia reaction genes of vascular endothelial growth factor(VEGF),erythropoietin (EPO) and hypoxia-inducible factor-1 alpha (HIF-1α)) at different times of rats that were induced epilepsy by kainic acid (KA),and analyze their correlation.Methods The epileptic rat model was established by intraperitoneal injection of kanic acid.The expression of VEGF,EPO and HIF-1α gene were detected by real-time fluorescent quantitative PCR assay with TaqMan probe in different times after intraperitoneal injection of KA.Results Compared with normal sodium (NS) group,the expression VEGF was higher at 12 h((8.38±1.27) ×10-3 ng/μl,P＜0.05)) and 24 h((8.30±5.08) ×10-3 ng/μl,P＜0.05)),EPO was higher at 12 h((8.42±0.90) × 10-5 ng/μl,P＜0.05)) and 48 h ((1.50±3.25) × 10-2 ng/μl,P＜0.01)) while the HIF-1α was higher at 24 h((2.11±0.21) × 10-2 ng/μl,P＜0.01)),48 h((1.50±0.33) × 10-2 ng/μl,P＜0.05))and 72 h((1.64±0.16) × 10-2 ng/μl,P＜0.01)).Furthermore,the expression of EPO showed significant correlations with HIF-1α and VEGF (r=0.573,0.471,P＜0.05),VEGF and HIF-1α had eminent correlations (r=0.803,P＜0.01).Conclusions The expression of VEGF,EPO and HIF-1α participate in the seizure procedure and there is certain correlation between the three genes.
