Effects of high iodine on mRNA expression of thyroid hormone receptor and protein kinase A and sodium iodide symporter in peripheral blood of patients with thyroid diseases during lacatation
10.3760/cma.j.issn.2095-4255.2015.09.005
- VernacularTitle:高碘对哺乳期甲状腺疾病患者外周血促甲状腺激素受体和蛋白激酶A及钠碘同向转运体mRNA表达的影响
- Author:
Liang XUE
;
Lixiang LIU
;
Da WEN
;
Hongmei SHEN
;
Fangang MENG
;
Xiaoye ZHANG
- Publication Type:Journal Article
- Keywords:
Thyroid diseases;
Women;
Thyroid hormone receptors;
Protein kinase A;
Sodium iodide symporter
- From:
Chinese Journal of Endemiology
2015;34(9):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of high iodine on mRNA expression of thyroid hormone receptor (TSHR),protein kinase A (PKA) and sodium iodide symporter (NIS) in peripheral blood of patients with thyroid diseases during lacatation.Methods A total of 99 breast-feeding women were selected as observation objects in Shanxi Province's sufficient iodine and high iodine areas,and they were divided into case group and control group according to whether suffer from thyroid disease.In high iodine areas,there were 21 patients and 19 healthy controls.In sufficient iodine areas,there were 30 patients and 29 healthy controls.Peripheral blood of all the observation objects was collected,and mRNA expression of TSHR,PKA and NIS was detected by real-time quantitative PCR.Results The case group [median (M):0.099,0.994] and the control group (M:0.240,0.738) in the high iodine areas were respectively compared with the case group (M:3.087,1.127) and the control group (M:1.823,0.842) in the sufficient iodine areas.The TSHR mRNA expression was significantly decreased (Z =-5.034,-4.010,all P ＜ 0.01);the PKA mRNA expression had a downward trend,and the difference was not statistically significant (Z =2.895,-0.343,all P＞ 0.05).The NIS mRNA expression of the case group in high iodine areas (M:0.485) was obviously lower than that of the the case group in sufficient iodine regions (M:2.680,Z=-3.311,P ＜ 0.01).The control group in high iodine areas (M:0.470) was compared with the control group in sufficient iodine areas (M:0.835),and the difference was not statistically significant (Z =-1.882,P ＞ 0.05).The NIS and the TSHR mRNA were positively correlated [correlation coefficient (r) =0.741,P ＜ 0.01];the NIS and the PKA mRNA was also positively correlated (r =0.293,P ＜ 0.01);but the TSHR mRNA was not significantly correlated with the PKA mRNA (r =-0.081,P ＞ 0.05).Conclusion Lactating women may have protected themselves and their babies through TSH-TSHR-cAMP-PKA signaling pathway that regulating iodine levels.
