Effects of silencing STIL by RNA interference on biological behaviors of gastric cancer cell line
10.3760/cma.j.issn.1673-4203.2015.10.010
- VernacularTitle:RNA干扰沉默STIL基因表达对胃癌细胞生物学行为的影响
- Author:
Ju WANG
;
Yong ZHANG
;
Xiaoping GAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
STIL;
RNA interference;
Cell proliferation;
Cell cycle;
Apoptosis
- From:
International Journal of Surgery
2015;42(10):684-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression pattern of STIL in gastric cancer and effect of silencing STIL on biological behaviors of SGC-7901 cell line.Methods The expression of STIL at mRNA and protein level were detected by qPCR and Western Blot,respectively.SiRNA against STIL was constructed and transfected into SGC-7901 cell line.The effect of Si-STIL on cell proliferation,cloning ability,cycle distribution and apoptosis was detected by MTT,colony-forming assay and flow cytometry,respectively.Results STIL was aberrantly expressed in gastric cancer compared with that in paratumor tissues.Downregulation of STIL level,inhibition of proliferation and cloning ability,increasing apoptosis and blocked cell cycle at S phase were observed after SGC-7901 cell line was treated with Si-STIL.Conclusion STIL was overexpressed in gastric cancer.STIL gene silencing effectively inhibited cell growth,transition of metaphase and promoted apoptosis of SGC-7901 cell line.
