High concentrations of urea induce human brain microvascular endothelial cell line to produce inflammatory cytokines
- VernacularTitle:高浓度尿素诱导人脑微血管内皮细胞系产生炎性因子
- Author:
Qi WANG
;
Hongkai WANG
;
Lijun LIU
;
Fei HE
;
Kejian WANG
;
Jinfang LI
;
Jianhua RAN
- Publication Type:Journal Article
- Keywords:
urea;
human brain microvascular endothelial cells;
TNF-α;
NO;
NF-κB
- From:
Basic & Clinical Medicine
2015;(2):152-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore high concentrations of urea-induced human brain microvascular endothelial cell line( HBMECs) to produce inflammatory cytokines and possible mechanism .Methods HBMECs were incubated in high concentrations of urea or mannitol ( as osmotic control ) for 3,6,12 and 24 hours.Expression of TNF-αand iNOS was observed by immunofluorescence .Western blot analysis was employed to assess the protein expressions of TNF-α, iNOS, COX-2, NF-κB/P65 and p-P65.NO concentration was determined by a commercial NO assay kit . Results Immunofluorescence showed high positive immunostaining of TNF-αand iNOS after incubation in high concentration of urea stimulued as compared with control group .The protein expressions of TNF-α, COX-2 and p-P65 were significantly increased at 3 and 6 hours after high urea treatment (P<0.01), and iNOS was continued to increase from 3 to 24 hours ( P<0.01 ) .Moreover , NO content was increased at 3 hours after high urea treatment ( P<0.05 ) .Conclusions High concentration of urea can induce HBMECs to produce inflammatory cytokines .
