miR-34a-5p inhibits the erythroid differentiation of K562 cells
- VernacularTitle:miR-34 a-5 p抑制K562细胞红系分化
- Author:
Hualu ZHAO
;
Wenjing BU
;
Yuxia LI
;
Di ZHAI
;
Xin WEN
;
Jia YU
- Publication Type:Journal Article
- Keywords:
miR-34a-5p;
K562;
c-MYB;
erythroid differentiation
- From:
Basic & Clinical Medicine
2015;(2):167-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of microRNA-34a-5p on erythroid differentiation of K562 cells.Methods K562 cells were transfected with the microRNA-34a-5p mimics and antisense inhibitors specifically targeting mi-croRNA-34a-5p, respectively.The effects of over-expression or knocking-down of microRNA-34a-5p were exam-ined by Quantitative RT-PCR.Flow cytometry was performed to detect specific surface marker of erythroid cells . The benzidine staining assay was used to access the differentiation of K 562 cells.Western blot was performed to de-tect miRNA targets.Results microRNA-34a-5p was down-regulated at the early stage of K562 erythroid differenti-ation.Over-expression of microRNA-34a-5p in K562 cells attenuates erythroid differentiation , in contrast, inhibi-tion of microRNA-34a-5p accelerates erythroid pheotypes in K562 cells.c-MYB was found to be the direct target of microRNA-34 a-5 p in erythroid cells .Conclusions microRNA-34 a-5 p regulates early erythroid differentiation of K562 cells via repressing c-MYB.
