CCK-8 decreases the expressions of CHOP/caspase-11/caspase-1 induced by LPS in mouse monocyte RAW 264.7 cells
- VernacularTitle:CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP／caspase-11／caspase-1的表达
- Author:
Wei LIU
;
Lin DUAN
;
Guangming XU
;
Feng YU
;
Xiaojing ZHANG
;
Ning SONG
- Publication Type:Journal Article
- Keywords:
cholecystokinin;
lipopolysaccharides;
C/EBP homologous protein;
caspase-11;
caspase-1
- From:
Basic & Clinical Medicine
2015;(2):178-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cells.Methods RAW264.7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times .CHOP and caspase-11 protein expressions were determined by West-ern blot , caspase-1 activity was analyzed by kit , IL-1βexpression level was analyzed by ELISA .Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264.7 cell, which were inhibited by pre-treatment of CCK-8 .Pre-treatment of devazepide inhibited the effects of CCK-8 significantly .The same effects were found in caspase-1 activity and IL-1βexpression .Conclusions CCK-8 exerts an anti-inflammatory effect by inhib-iting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cell.CCK 1R may contribute to the anti-inflammatory effect of CCK-8.
