miR-141 suppresses cell proliferation by target down-regulating EphA2 in human oral squamous cell carcinoma
- VernacularTitle:miR-141通过靶向下调EphA2表达而抑制人口腔鳞状细胞癌细胞系的增殖
- Author:
Zhe HAN
;
Lei LI
;
Xiaoqing ZHOU
- Publication Type:Journal Article
- Keywords:
oral squamous cell carcinoma;
miR-141;
EphA2;
proliferation
- From:
Basic & Clinical Medicine
2015;(2):213-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of miR-141 on proliferation of human oral squamous cell carcinoma and target relationship between miR-141 and EphA2 .Methods pcDNATM6.2-GW-pre-miR-141 was constructed and identified by qRT-PCR.EphA2-WT and EphA2-MT sequences were respectively cloned into pmirGLO plasmid . The potential proliferation function of miR-141 on CAL27 cells was analyzed by MTT .The target relationship be-tween miR-141 and EphA2 was identified by Dual-Luciferase Assay System , qRT-PCR and Western blot .Results We constructed successfully the recombinant plasmids , including pcDNATM6.2-GW-pre-miR-141, pmirGLO-E-phA2-WT and pmirGLO-EphA2-MT, and the transfection efficiency of pre-miR-141 was increased in CAL27 cells compared to control group(P<0.001).miR-141 could suppress the proliferation of CAL27 cells(P<0.05). Furthermore, a significant reduction of luciferase activities of CAL27 cells co-transfected with pre-miR-141 and EphA2-WT(P<0.001).The mRNA(P<0.001) and protein expression levels of EphA2 were decreased in CAL27 cells transfected with pre-miR-141 .Conclusions Overexpression of miR-141 may suppress cell prolifera-tion by targeting at EphA2 in CAL27 cells.
