Intermedin1-53 inhibits collagen synthesis induced by angiotensinⅡin cadiac fibroblasts of rats
- VernacularTitle:Intermedin1-53抑制血管紧张素Ⅱ诱导的大鼠心肌成纤维细胞合成胶原
- Author:
Xiangquan KONG
;
Yan SUI
;
Jinghan JIANG
;
Shouming XU
;
Hui ZHANG
- Publication Type:Journal Article
- Keywords:
Intermedin1-53;
cardiac fibroblast;
angiotensin Ⅱ;
transforming growth factor-β
- From:
Basic & Clinical Medicine
2015;(2):187-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate collagen metabolism modulation of Intermedin 1-53 ( IMD1-53 ) on angiotensin Ⅱ( AngⅡ)-induced rat cardiac fibroblasts .Methods SD neonatal rat cardiac fibroblasts were cultured and divided them into control group, AngⅡ +different concentrations IMD1-53 groups.ⅠandⅢcollagen expression in cardiac fibroblasts were measured by Westem blot , IMD1-53 receptor-like receptor ( calcitonin receptor-like receptor , CRLR) and transforming growth factor-β( TGF-β) mRNA expression were exammed by real-time PCR.Results IMD1-53 significantly inhibited AngⅡ-induced collagen synthesis in cardiac fibroblasts , and this effect was more ob-vious with the increase of IMD 1-53 ( P<0.01 ,P<0.05 ) .Similar phenomenon was recorded in TGF-βexpression in cardiac fibroblasts ( P<0.05 ) .Conclusions IMD1-53 inhibited collagen synthetic in cardiac fibrosis induced by AngⅡ, down-regulated TGF-βexpression.These may relate to IMD1-53 anti myocardial fibrosis.
