Osthole promotes differentiation into neurons and reduces neuronal apoptosis via Wnt/β-catenin signaling pathway in APP transduced neural stem cells
10.3969/j.issn.1001-1978.2015.11.009
- VernacularTitle:蛇床子素通过Wnt/β-catenin信号通路促进转染APP基因的神经干细胞分化为更多神经元且减少神经元凋亡
- Author:
Yingjia YAO
;
Liang KONG
;
Yanan JIAO
;
Shaoheng LI
;
Zhenyu TAO
;
Yuhui YAN
;
Jingxian YANG
- Publication Type:Journal Article
- Keywords:
Alzheimer′s disease;
neural stem cells;
proliferation;
differentiation;
neurons;
osthole;
Wnt/β-catenin signaling pathway
- From:
Chinese Pharmacological Bulletin
2015;(11):1516-1523
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of osthole ( Ost) on the ability of proliferation and differentiation in APP transduced neural stem cells( NSCs) , and neu-ronal apoptosis, in order to find related mechanism. Methods A model of Alzheimer′s disease( AD) cells was successfully established by transducing APP gene into NSCs in vitro. The ability of proliferation and dif-ferentiation was tested by staining. The viability of NSCs was determined by using CCK-8 assay. The cell apoptosis was tested by Hoechst 33258 staining. The expression of GSK-3β and β-catenin mRNA was deter-mined by RT-PCR. The expression of GSK-3β and β-catenin protein was determined by Western blot. Re-sults The ability of proliferation had increased by 10 . 24% with Ost treatment, compared with APP group. The ability of differentiation had increased by 6 . 74%with Ost treatment, compared with APP group. The vi-ability of NSCs had increased and cell apoptotic rate had decreased significantly. From the results of RT-PCR and Western blot, we could find the expression of GSK-3βmRNA and protein had decreased, and the ex-pression of β-catenin mRNA and protein had increased significantly, compared with APP group. Conclusion Ost could enhance the ability of proliferation and dif-ferentiation into more neurons of NSCs transducing APP gene, and reduce neuronal apoptosis. It might be relat-ed with activiting Wnt/β-catenin signaling pathway.
