Effects of Morroniside on Expression of Vascular Endothelial Growth Factor and Fibroblast Growth Factor-2 in Rats after Focal Cerebral Ischemia-reperfusion
10.3969/j.issn.1006-9771.2015.11.001
- VernacularTitle:莫诺苷对局灶性脑缺血再灌注大鼠血管内皮生长因子和成纤维生长因子-2表达的影响
- Author:
Deyu GUO
;
Fangling SUN
;
Renping WEI
;
Tingting LIU
;
Hua CHENG
;
Houxi AI
;
Xin TIAN
;
Zixin ZHU
;
Wenrong ZHENG
;
Yufeng WANG
;
Wen WANG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion;
morroniside;
angiogenesis;
vascular endothelial growth factor;
fibroblast growth factor-2;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(11):1241-1244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of morroniside on the expression of vascular endothelial growth factor (VEGF) and fi-broblast growth factor-2 (FGF-2) in rat cortex after focal cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were ran-domly divided into sham group, model group, morroniside-low group (30 mg/kg), morroniside-middle group (90 mg/kg) and morroni-side-high group (270 mg/kg). Middle cerebral arteries of rats were occluded for 30 minutes with Longa's method and re-perfused. The ex-pression of VEGF and FGF-2 in the ischemic ipsilateral cortex was detected with Western blotting 7 days after reperfusion. Results The ex-pression of both VEGF and FGF-2 increased in the ischemic ipsilateral cortexin in all the ischemic groups compared with the sham group (P<0.05). The expression of VEGF further increased in a dose-dependent manner in all the morroniside groups compared with that of model group (P<0.05), and the expression of FGF-2 increased in the morroniside-high group (P<0.001). Conclusion Morroniside could increase the expression of VEGF and FGF-2 after ischemia-reperfusion, which might promote angiogenesis.
