Effects of Electroacupuncture on Activation of Microglia in Peri-infarct Cortex of Cerebral Ischemia-reperfusion Injury Rats
10.3969/j.issn.1006-9771.2015.11.003
- VernacularTitle:电针对缺血再灌注损伤大鼠缺血周围区皮质小胶质细胞活化的影响
- Author:
Xian WANG
;
Jia HUANG
;
Weilin LIU
;
Hao SHANGGUAN
;
Yi ZHENG
;
Lulu WANG
;
Yunjiao LIN
;
Jing TAO
;
Lidian CHEN
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury;
electroacupuncture;
microglia;
inflammation;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(11):1251-1255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of electroacupuncture on activation of microglia in peri-infarct cortex after cerebral isch-emia-reperfusion in rats. Methods 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and electroacupuncture group (n=12). The latter two groups were occluded the left middle cerebral arteries with modified Longa's method for 2 hours and reperfused. The electroacupuncture group received electroacupuncture at Quchi (LI11) and Zusanli (ST36) acupoints for 3 days. The nerve cell damage in peri-infarct cortex was observed with HE staining, while the expression of ED1 was determined with immunohisto-chemical staining, and the expression of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and IL-6 were determined with Western blotting. Results The neurological deficits score improved significantly in the electroacupuncture group (P<0.05), with less nerve cell dam-age, less number of ED1 positive microglia (P<0.05) and less levels of TNF-α, IL-1βand IL-6 (P<0.05), compared with the model group. Conclusion The electroacupuncture at Quchi (LI11) and Zusanli (ST36) acupoints can protect brain from ischemia-reperfusion injury, which might be associated with inhibiting the microglial activation and proinflammatory response in peri-infarct cortex.
