Notch signaling pathway regulates epithelial-mesenchymal transition and affects the invasiveness and drug resistance of bladder cancer
- VernacularTitle:Notch信号通路调控上皮-间质转化影响膀胱癌侵袭性和耐药性
- Author:
Zhihuan LIU
;
Yibing WANG
;
Gongxian WANG
;
Liang HUANG
;
Bin LANG
;
Xiaoyuan XU
;
Bin FU
- Publication Type:Journal Article
- Keywords:
bladder cancer;
Notch signal;
epithelial-mesenchymal transition;
γ-secretase inhibitor
- From:
Basic & Clinical Medicine
2015;(2):145-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of notch signaling pathway on drug resistance and invasiveness of bladder cancer .Methods We observed the changes of growth and morphology of bladder cancer T 24 , 5637 and J82 cells which treated for 48 hours using γ-secretase inhibitor by inverted microscope .The mRNA and protein lev-els of the EMT molecular markers , including E-cadherin , N-cadherin , vimentin and Alpha-smooth muscle actin were examined by RT-PCR and Western blot in bladder cancer cells;Detected the changes of drug resistance and invasion respectively by MTT and Transwell in bladder cancer cells .Results After completely blocking the Notch signaling pathway , the inverted microscope showed that bladder cancer cells became smaller and more disperse ;RT-PCR and Western blot showed the mRNA and protein levels of E-cadherin were up-regulated ( P<0.05 ) , contrast , N-cadherin , vimentin and Alpha-smooth muscle actin were down-regulated ( P<0.05 ); The prolifera-tion of bladder cancer cells were significantly inhibited by MTT test;The number of through microporous membrane cells significantly decreased ( P<0.05 ) shown by Transwell test .Conclusions The Notch signaling pathway is completely blocked that nhibites proliferation and EMT of bladder cancer cells , reduces drug resistance and inva-sion in bladder cancer cells .It suggests that drug resistance and invasiveness of bladder cancer can be changed through EMT which is regulated through notch signaling pathway .
