HMGB1 RNAi inhibits TGF-β1 induced epithelial-mesenchymal transition in A 549 cells
- VernacularTitle:HMGB1 RNAi抑制TGF-β1诱导的A549细胞系EMT
- Author:
Lin CAI
;
Zhiyan RUAN
;
Jun XU
- Publication Type:Journal Article
- Keywords:
RNAi;
high mobility group B1;
α-smooth muscle actin;
epithelial-mesenchymal transition
- From:
Basic & Clinical Medicine
2015;(2):183-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of HMGB 1 in epithelial-mesenchymal transition .Methods Specific siRNA of HMGB1 was designed and synthesized .Cultured typeⅡalveolar epithelial cell line-A549 cells were divided into 4 groups:1)control group, 2)model group induced by TGF-β1, 3)HMGB1 RNAi group, 4)RNAi negative con-trol group .Cellular morphology changes were observed by phase-contrast microscope .HMGB1 andα-SMA expression in A549 cells was detected by RT-PCR and Western blotting respectively .Results mRNA and protein expression of HMGB1 andα-SMA in TGF-β1 group increased significantly than that in control group (P<0.01).HMGB1,α-SMA mRNA and protein expression in siRNA-treated cells decreased significantly as compared with that in TGF -β1 group (P<0.01).Conclusions HMGB1 may be involved in the TGF-β1 induced EMT.
