Application analysis of time-resolved fluorescence immunoassay technology in syphilis specific antibody screening
10.3969/j.issn.1673-4130.2015.21.016
- VernacularTitle:时间分辨荧光免疫技术在梅毒特异性抗体筛查中的应用研究
- Author:
Wei DAI
;
Shiyun LI
;
Dejun XIAO
;
Ting LIU
;
Jiuchang XIAO
;
Jing LI
- Publication Type:Journal Article
- Keywords:
syphilis specific antibody;
TRFIA;
TP-ELISA;
TPPA;
comparative analysis
- From:
International Journal of Laboratory Medicine
2015;(21):3117-3118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of time‐resolved fluorescence immunoassay (TRFIA) in the detection of specific antibody of syphilis .Methods Specific antibody of syphilis was detected in serum samples of 240 cases of syphilis and 150 healthy subjects by TRFIA ,Treponemal pallidum particle agglutination(TPPA) and Treponemal pallidum enzyme linked immu‐nosorbent assay(TP‐ELISA) .The sensitivity ,specificity and positivity of these three methods were compared .Results The sensi‐tivity of TRFIA ,TP‐ELISA ,TPPA were 100 .00% ,98 .75% and 97 .92% ,without significantly differences(P>0 .05) ,and the spe‐cificity were 99 .33% ,98 .67% and 100 .00% .The false positive rate of TRFIA was 0 .67% ,and the false negative rate was 0 .00% . The false positive rate of TP‐ELISA was 1 .33% ,and the false negative rate was 1 .25% .False positive rate and false negative rate of TRFIA were lower than TP‐ELISA(P<0 .05) .Conclusion TRFIA could be with high sensitivity and specificity in syphilis spe‐cific antibody test ,and could be used for routine screening of syphilis specific antibody .
