Effect of Yangxue Qingnao Granule on Ultrastructures and Expression of p38 Mitogen Activated Protein Kinases in CA1 Area of Hippocampus of Vascular Dementia Rats
10.3969/j.issn.1006-9771.2015.11.002
- VernacularTitle:养血清脑颗粒对血管性痴呆大鼠海马CA1区超微结构及p38丝裂原活化蛋白激酶蛋白表达的影响
- Author:
Yunkun SAN
;
Jinxia ZHANG
;
Bin LIU
;
Ying LIU
;
Shiying LI
- Publication Type:Journal Article
- Keywords:
vascular dementia;
Yangxue Qingnao Granules;
p38 mitogen activated protein kinases;
ultrastructure;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(11):1245-1250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Yangxue Qingnao Granule on the ultrastructures and expression of p38 mitogen activated protein kinases (p38MAPK) in CA1 area of hippocampus in vascular dementia rats. Methods 180 Sprague-Dawley rats were randomly di-vided into sham group, vascular dementia model group (model group) and Yangxue Qingnao Granule treatment group (treatment group). The vascular dementia was modeled with modified Pulsineli's four-vessel occlusion. The ultrastructure of CA1 area was observed with trans-mission electron microscope, while the expression of p38MAPK in CA1 area was detected with immunohistochemstry and Western blotting 1, 2, 4 and 8 weeks after modeling. Results In the model group, pyknosis, nuclear dissolution, heterochromatin margination and mitochon-dria swelling were found in most of the neurons in CA1. In the treatment group, the distribution of chromatin was well-proportioned, and mi-tochondrion and other organelle were normal. In the model group, the expression of p38MAPK increased at each time point compared with the sham group (P<0.01), and peaked 4 weeks after modeling, and decreased in the treatment group compared with the model group (P<0.01). Conclusion Yangxue Qingnao Granule can improve the ultrastructure of neuronal in CA1 area of hippocampus of vascular dementia rats, which may relate with the inhibition of the expression of p38MAPK.
