The clinical value of miR-127-3p expression in plasma of patients with breast cancer
10.3760/cma.j.issn.1009-9158.2015.10.008
- VernacularTitle:乳腺癌患者血浆中miR－127－3p表达的临床价值
- Author:
Meihong LU
;
Wei SHI
;
Hui CONG
;
Lingling XIE
;
Rongrong JING
;
Jiahui MAO
;
Shaoqing JU
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
microRNAs;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2015;(10):682-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical value of miR-127-3p in plasma of patients with breast cancer .Methods 80 cases of breast patients , 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited .A real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR ) method for detecting miR-127-3p was established; Liner, reproducibility, and specificity were evaluated.In addition, correlations between the relative expression of plasma miR-127-3p and the concentrations of CEA and CA 153 were assessed.the relationship of miR-127-3p expression and clinicopathological features was further determined by Mann-Whitney test.Results The method for detection of plasma miR-127-3p was established.The relative plasma expression of miR-127-3p in breast patients [ 10.561 ( 5.424 -16.465 ) ] was significantly higher than that in benign breast tumor patients [3.015 (1.987-5.035)] (P=0.000 6) and healthy controls [2.375 (1.173-4.370)] (P=0.000 2).However, there was no significant difference between benign tumor patients and healthy control group (P=0.143).Positive relationship was found between the relative expression of miR-127-3p and the concentration of CA153 (R2 =0.457, P=0.003).The area under the ROC curve (AUC) of miR-127-3pwas 0.763, which was higher than that of CEA and CA 153.No significant difference was found between plasma miR-127-3p expression and clinicalpathological features including tumor size , differentiation and tumor node metastasis stage (P>0.05).Conclusions miR-127-3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer .
