Expression of transforming growth factor beta3 in meniscus development
10.3969/j.issn.2095-4344.2015.33.006
- VernacularTitle:转化生长因子β3在半月板发育过程中的表达
- Author:
Jian WANG
;
Yu SUN
;
Xizhuang BAI
- Publication Type:Journal Article
- Keywords:
Transforming Growth Factor beta3;
Menisci,Tibial;
Knee Joint
- From:
Chinese Journal of Tissue Engineering Research
2015;(33):5274-5278
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Since the meniscal blood supply is only in the red zone, the synovial fluid becomes the main nutrition source of the meniscus. As its poor self-healing ability, the meniscus is almost impossible to heal in non-blood supply regions. OBJECTIVE:To investigate the expression and characteristics of transforming growth factor-β3 in the rat meniscus during postnatal development. METHODS:Meniscus tissues of Wistar rats with different ages (1, 7, 14, 21, 28, 35 days) were adopted, and immunohistochemistry and RT-PCR methods were performed to detect the location and expression pattern of transforming growth factor-β3 within the meniscus. RESULTS AND CONCLUSION: Immunohistochemistry results revealed that the expression of transforming growth factor-β3 started from the joint capsule, and gradualy extended to the red zone, gray zone, and white area;RT-PCR results showed the mRNA expression of transforming growth factor-β3 in the rat meniscus at different ages, and during development process gradualy increased until adult stage (rat weight > 100 g), which remained relatively stable.
