Influence of bisphosphonates and cisplatin on MG-63 cell lines culturedin vitro
10.3969/j.issn.2095-4344.2015.33.016
- VernacularTitle:二膦酸盐与顺铂对体外MG-63细胞株生长影响的比较
- Author:
Hasimu MAIMAITIAILI
;
Renbing JIANG
;
Jingping BAI
- Publication Type:Journal Article
- Keywords:
Diphosphonates;
Cisplatin;
Osteosarcoma
- From:
Chinese Journal of Tissue Engineering Research
2015;(33):5327-5331
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that bisphosphonates can act on osteosarcoma cels, but the comparative analysis of bisphosphonates and cisplatin, a traditional first-line chemotherapy drug, is rarely reported. OBJECTIVE:To investigate the differences between bisphosphonates and cisplatin to inhibitin vitroproliferation of osteosarcoma cels and induce cel apoptosis. METHODS: Subcultured MG-63 cel lines were intervened with different concentrations of bisphosphonates and cisplatin, respectively, and cels with no treatment served as negative controls (blank group). Cel inhibition rate was detected using MTT method, and cel morphology was observed using fluorescent staining. RESULTS AND CONCLUSION:After intervention with different concentrations of bisphosphonates and cisplatin for different time, the growth inhibition rate of MG-63 cels was significantly higher as compared with the blank group (P < 0.05). But there was no difference in the growth inhibition rate between the bisphosphonates and cisplatin groups at 24, 48 and 72 hours (P > 0.05). These findings indicate that bisphosphonates has obvious inhibitory effect on the growth of osteosarcoma celsin vitro, and its inhibitory effect is similar to that of cisplatin.
