Development and applications of inexpensive genotyping methods
10.3321/j.issn:1000-5048.2009.03.001
- VernacularTitle:低成本基因多态性检测方法及其应用研究
- Author:
Guohua ZHOU
;
Qinxin SONG
- Publication Type:Journal Article
- Keywords:
single nucleotide polymorphism( SNP);
genotyping platforms;
application
- From:
Journal of China Pharmaceutical University
2009;40(3):193-199
- CountryChina
- Language:Chinese
-
Abstract:
Single nucleotide polymorphism( SNP) is a major source of genetic differences, and the number of SNP is extremely large. Consequently, a high-throughput, rapid and cost-effective technique is essential for SNP typing. Recently, a set of solutions aimed directly at the bottlenecks in SNP detection were put forward, which including direct PCR from human whole blood, adapter-ligation mediated allele-specific amplification, gene polymorphism detection in one tube, microplate array parallel gel electrophoresis and microchip electrophoresis. These methods have been successfully applied in pharmacogenomics, disease-related research, herbal medicine identification and quality control. The results showed that the advantages of the genotyping platforms are highly specific, sensitive, easy to operate and inexpensive with a good prospect of application. The principles and applications of the genotyping platforms in detail were described in this paper.
