Enzymatic degradation of marine polysaccharide YCP and analysis of YCP-derived fragments
- VernacularTitle:海洋真菌多糖YCP的酶法降解及其产物分析
- Author:
Wei YAN
;
Min REN
;
Wenbing YAO
;
Lei JIN
;
Xiangdong GAO
- Publication Type:Journal Article
- Keywords:
YCP;
polysaccharide;
enzymatic degradation;
α-amylase;
immunological activity
- From:
Journal of China Pharmaceutical University
2010;41(1):76-80
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To explore the relationship between structure and immunological activity of marine polysaccha-ride YCP,the physicochemical property and immunological activity of the YCP-derived fragment were studied.Methods: YCP was hydrolyzed by α-amylase from human saliva.The hydrolysate was purified to obtain an polysaccharide fragment by gel filtration chromatography.The physicochemical properties of this YCP-derived fragment was characterized by HPLC,FT-IR and TLC.In addition,changes of phagocytic activity,production of reactive nitrogen and macrophage binding were investigated.Results: The relative molecular weight of YCP-de-rived fragment was approximately 6.6 × 10~3.The monosaccharide composition and FT-IR of the YCP-derived frag-ment were identical to YCP.No significant effect of the YCP-derived fragment on NO production and murine mac-rophage phagocyte were observed.And this fragment was not able to compete the binding between YCP and mac-rophages.Conclusion: The remarkable decrease of immunological activity of YCP-derived fragments degraded byα-amylase of human saliva suggests that the complete structure and high molecule weight of YCP are essential for its immuno-modulatory activity.
