Multiresidue determination of fluoroquinolones in eggs by solid-phase extraction-LC-MS/MS
- VernacularTitle:固相萃取-LC-MS/MS测定鸡蛋中氟喹诺酮类药物残留
- Author:
Yuan TIAN
;
Zunjian ZHANG
;
Jing LI
;
Wenjing LI
;
Yun CHEN
- Publication Type:Journal Article
- Keywords:
fluoroquinolones;
egg;
muhiresidue;
LC-MS/MS;
solid-phase extraction
- From:
Journal of China Pharmaceutical University
2010;41(1):60-65
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To establish an analytical method for the simultaneous determination of norfloxacin,ofloxacin,pefloxacin,ciprofloxacin,lomefloxacin,danofloxacin,enrofloxacin,sarafloxacin and difloxacin in eggs using solid-phase extraction-LC-MS/MS.Methods: Egg samples were deproteinized with acetonitrile,followed by defatting with hexane.Then the samples were processed by solid-phase extraction and analyzed by LC-MS/MS using an electrospray source.The separation was carried out on a Shimadzu Shim-pack VP-ODS C_(18) column,with a mobile phase consisting of acetonitrile-0.1% formic acid(13: 87).Results: The validated method was proved to be of high specificity,accuracy and sensitivity.Conclusion: The established method is suitable for the routine residual monitoring of fluoroquinolones.
