Combination of high fat-hgih sucrose diet and chronic stress promotes insulin resistance in rats
10.3321/j.issn:1000-5048.2009.04.018
- VernacularTitle:高脂高糖饲料喂养结合慢性应激促进大鼠胰岛素抵抗
- Author:
Lili TAN
;
Hongshuang SUN
;
Shengrong XIE
;
Shujia KONG
;
Yuan WANG
;
Jihua FU
- Publication Type:Journal Article
- Keywords:
chronic stress;
insulin resistance;
high fat-high sucrose diet;
hyperinsulinemic englycemic clamp experiment;
synergistic effect
- From:
Journal of China Pharmaceutical University
2009;40(4):380-384
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To observe the influence of high fat-high sucrose diet and chronic stress on insulin resistance. Methods: Male rats were divided into four groups: control, high fat-high sucrose diet( HFSD), chronic stress( CS), high fat-high sucrose diet plus chronic stress( HFSD + CS). After feeding the animals for 10 weeks, fat, glucose and insulin concentrations in blood and PPAR-α mRNA expression in liver were examined, and glu-cose infusion rate was detected by a hyperinsulinemic euglycemic clamp experiment. Results: Insulin resistance was observed in all three treated groups, showing the highest in the HFSD + CS group. Dyslipidemia, hypergly-cosemia, hyperinsulinism and the decrease of PPAR-α mRNA expression in liver were also shown in all treated groups. There was an obvious interaction of insulin resistance, hyperglycosemia and high FFA between high fat-high sucrose diet and chronic stress. Conclusion: Combination of high fat-high sucrose diet and chronic stress could promote the development of insulin resistance, which is likely due to the high level of serum FFA.
