Inhibitory effects and mechanism of ursolic acid on the proliferation of rat aortic smooth muscle cells
- VernacularTitle:熊果酸抑制大鼠血管平滑肌细胞增殖的作用及其机制
- Author:
Jianmei WANG
;
Min XIANG
;
Pingping WU
;
Yaqin ZHANG
- Publication Type:Journal Article
- Keywords:
ursolic acid;
vascular smooth muscle cell(VSMC);
p38MAPK;
c-fos
- From:
Journal of China Pharmaceutical University
2010;41(1):66-69
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To observe the effect of ursolic acid(UA)on the proliferation of rat vascular smooth muscle cell(VSMC)induced by high-level glucose and explore its relationship with p38MAPK signal transduction pathway.Methods: The proliferation of VSMC induced by high-level glucose(25 mmol/L glucose)was adopted as model,the inhibition of UA on the proliferation of VSMC was measured by MTT assay,and the expression lev-els of phospho-p38MAPK was detected by cell-based ELISA as well as the expression of c-fos protein was exam-ined by SABC method.Results: UA(20 μmol/L and 40 μmoL/L)inhibited glucose-induced proliferation of VSMC(P <0.05).Compared with the group subjected to glucose induction,UA decreased the expression levels of phosphorylated p38MAPK(P < 0.05),and also inhibited c-fos expression.Conclusion: UA suppressed glucose induced proliferation of VSMC,which might be related to the suppression of the activation of p38MAPK signal transduction pathway,and thereby down-regulated c-fos expression.
