Application of internal standard correction method in the simultaneous determination of multi-components of Fructus Gardeniae
- VernacularTitle:内标校正法在中药栀子多成分同时定量分析中的应用
- Author:
Zhiming BI
;
Xiaoqin ZHOU
;
Dan TANG
;
Qian WU
;
Lianwen QI
;
Ping LI
- Publication Type:Journal Article
- Keywords:
internal standard correction method;
Fructus Gardeniae;
quantitative analysis
- From:
Journal of China Pharmaceutical University
2010;41(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To establish a simple and convenient quality evaluation method for Fructus Gardeniae(FG).Methods: The internal standard correction factors(f_(km))of four crocetin derivatives to crocin-1 were established and the system suitability was tested.The content of crocin-1 was determined by external standard method,the contents of other crocetin derivatives were calculated by the content of crocin-1 and their f_(km).Results: There was no significant difference between the test results obtained by the methods oftheoretical calculation by f_(km)androutire determination technique.Conclusion: The internal standard correction method(the method oftheoreti-cal calculation by f_(km))is practical and feasible in the quantitative analysis of Frnctus Gardeniae.
