Quality analysis of 3 species of Radix Paeoniae Alba cultivated in Bozhou by HPLC and LC-MS

VernacularTitle:HPLC、LC-MS检测3种亳白芍栽培品种的质量
Author: Yanan TIAN ; Jie YANG ; Yanna LIU ; Qiang WANG
Publication Type:Journal Article
Keywords: Radix Paeoniae Alba in Bozhou; cultivated species; HPLC; LC-MS; paeoniforin
From: Journal of China Pharmaceutical University 2009;40(3):227-231
CountryChina
Language:Chinese
Abstract: Aim: To establish the HPLC and LC-MC methods for the quality analysis of the different species of Radix Paeoniae Alba cultivated in Bozhou. Methods: The LC-MS method was applied to analyze the chemical composition of the cultivated species, the HPLC method was applied to determine the contents of paeoniforin, ben-zoic acid and paeonivayin in crude drugs and processing products. Results: 10 characteristic peaks were identified by LC-MS with reference standards. The significant difference was observed in the chemical compositions of 3 cultivated species. The significant difference was observed in the chemical compositions of 3 cultivated species. The content of paeoniflorin was increased significantly after traditional processing. The results indicated the 4-year-old pubang and 5-year-old xiantiao are the best cultivated species. Conclusion: The methods are simple, rapid and precise to assess and control the quality of different species of Radix Paeoniae Alba cultivated in Bozhou.