Protective effects of the specific cyclooxygenase-2 inhibitor on microglia activation-mediated neuronal damage
10.3760/cma.j.issn.1674-6554.2015.09.004
- VernacularTitle:环氧合酶2特异性抑制剂对小胶质细胞激活介导的神经元损伤的保护作用
- Author:
Peng LI
;
Guangmin XU
;
Xue YANG
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase 2 inhibitors;
Microglia;
Hippocampus;
Neurons
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(9):784-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of NS398,a specific cyclooxygenase-2 inhibitor,on microglial activation-mediated neuronal damage.Methods The microglia of neonatal SD rat were isolated and cultured in the medium containing 1 μg/ml of LPS.The morphological changes of microglial were observed.The IL-1β and TNF-α levels were detected by Western blot in LPS group (group L) and control group (group C).The hippocampal neurons of neonatal SD rats were cultured in the control medium (group C),LPS-activated microglial conditioned medium (group L) and LPS-activated microglial conditioned medium with NS398 (group N),respectively.The survival rate of neurons were detected by MTT.The respiration of hippocampus neurons was determined by detecting the ratio of lactic acid/pyruvic acid.Results LPS-induced microglial activation was characterized by the morphological change and increased secretion of IL-1β and TNF-α.The survival rate of neurons cultured by microglia activated conditioned medium was 64.37％,while the group N was 80.25％ (P＜0.01).In group L,the ratio of lactic acid/pyruvic acid (27.34±8.53) was significantly higher than that of group N (20.32±6.05,P＜0.01) and group C (14.95±4.72,P＜0.01).Conclusion NS398,a specific cyclooxygenase-2 inhibitor,has a protective effect on rat hippocampus neuron damaged by activated microglia.
