Comparison of polymorphism of MIF-173 gene locus with Henoch-Scholein purpura of children between the Han nationality and Moggol nationality
10.3760/cma.j.issn.1673-4408.2015.05.021
- VernacularTitle:蒙汉族儿童过敏性紫癜MIF-173位点基因多态性的分析比较
- Author:
Yuheng ZHANG
;
Peng LI
- Publication Type:Journal Article
- Keywords:
Macrophage migration inhibitory factor;
Henoch-Schonlein purpura;
Moggol nationality;
Han nationality;
Henoch-Schonlein purpura nephritis
- From:
International Journal of Pediatrics
2015;42(5):559-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study gene polymorphisms (CC, CG, G-G) of macrophage migration inhibitory factor (MIF) in children of the Han nationality and Moggol nationality and to find that whether MIF-173 gene position is associated with HSP of the Han nationality and Moggol nationality, and differences between the Han nationality and Moggol nationality in MIF-173 gene position.Methods All cases were enrolled in Inner Mongolia Medical University Affiliated Hospital and Inner Mongolia International Mongolian hospital from December 2012 to December 2013.There are 105 cases of the Han nationality and Moggol nationality with HSP and control groups, Aged from 1 to 14 years old.There are four groups: Moggol nationality with HSP group (25 cases) and the group of the Han nationality with HSP (25 cases), Moggol nationality control group (25 cases) and the Han nationality control group (25 cases).Each sample was drawed from children's venous blood 2ml for inspection.We used DNA extraction kit to extract all DNA and used the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect the polymorphism of MIF-173 gene loci.SPSS17.0 statistical software was used for analysis.Results MIF-173 gene position could be detected out three genotypes, GG, CG and CC type.Compared to the group of the Han nationality with HSP and the Han nationality control group, there is mostly MIF-173 CC gene type.the difference is significant statistically in these two groups (x2 =10.46, P ＜0.05).Compared to Moggol nationality with HSP group and Moggol nationality control group, there was mostly MIF-173 CC gene type.The difference is significant (x2 =6.61, P ＜ 0.05).Conclusion MIF may play an important role in the occurrence and development of HSP.There were no significant differences between Moggol nationality with HSP group and the Han nationality with HSP group (x2 =2.299, P＞0.05).
