Analysis and verification of gastric cancer metastatic lymphonodus associated long non-coding RNA

VernacularTitle:胃癌淋巴结转移相关长链非编码RNA的鉴定及验证
Author: Ziguo YANG ; Xiaobo GUO ; Yulong SHI ; Changqing JING ; Leping LI
Publication Type:Journal Article
Keywords: Stomach neoplasms; Lymphatic metastasis; Microchip analytical procedures; Long non-coding RNAs
From: Chinese Journal of General Surgery 2015;30(10):800-803
CountryChina
Language:Chinese
Abstract: Objective To analyze and verify the expression profiles of long non-coding RNAs (lncRNAs) in gastric cancer (GC) metastatic lymphonodus.Methods Microarray analysis was performed in 3 GC positive lymphonodus and 1 normal lymph node with Agilent Array platform to measure the expression levels of lncRNAs and mRNAs and to investigate the expression differences of lncRNAs in GC metastatic lymphonodus and normal lymphonodus, and hierarchical clustering used to screen out the differently expressed lncRNAs.15 up-regulated lncRNAs and 15 down-regulated lncRNAs were randomly chosen and RT-PCR was used to verify the expression differences.Results Comparing with normal lymphonodus, 353 lncRNAs and 547 mRNAs are up-regulated, but 464 lncRNAs and 562 mRNAs are down-regulated in GC metastatic lymphanodus as 6 times or more variation was found.The expressions of lncRNA OR3A4, LOC84740, FCGR1C and C21orf 96 were increased in GC metastatic lymphonodus, but lncRNA MSTO2P, LOC344595, TUG1, TYW3 and KRT8P10 decreased.Conclusions LncRNAs are aberrantly expressed in GC metastatic lymphonodus.