HM13 expression in gastric cancer and the correlation with invasion and metastasis of the tumor
10.3760/cma.j.issn.1007-631X.2015.09.017
- VernacularTitle:次要组织相容性抗原13在胃癌中的表达及其与侵袭转移的关系
- Author:
Yichao YAN
;
Zhanlong SHEN
;
Kewei JIANG
;
Yingjiang YE
;
Shan WANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Minor histocompatibility antigens;
Heme oxygenase (Decyclizing);
Neoplasm metastasis;
Prognosis
- From:
Chinese Journal of General Surgery
2015;30(9):726-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of ininor histocompatibility antigen 13 (HM1 3) in gastric carcinoma and the relationship with clinicopathological parameters and prognosis.Methods The expression of HM 13 was detected by immunohistochemistry in a total of 90 pairs of paraffin-embedded gastric cancer tissue specimens and corresponding paraneoplastic tissues.The correlation between clinicopathological parameters and the expression of HM13 in gastric carcinoma were also analyzed.Downstream gene heme oxygenase-1 (HO-1) expression was detected by qRT-PCR after HM13 gene was interfered.Results High expression of HM13 protein was observed in 47％ gastric carcinoma compared with that in 61％ corresponding paraneoplastic tissues (x2 =3.78,P =0.052).HM13 expression has positive correlation with pathological TNM stage (x2 =5.022,P =0.025) and distant metastasis (P =0.033),but not with age (x2 =0.832,P =0.362),gender (x2 =0.779,P =0.378),tumor size (x2 =0.804,P =0.370),tumor differentiation (x2 =0.430,P =0.512),gross types (x2 =2.069,P =0.150),tumor stromal invasion (x2 =0.167,P =0.683) and lymph node status (x2 =0.396,P =0.529).The patients with low HM13 expression had worse overall survival [(OS):(30 ± 5) months vs.(47 ± 5) months,x2 =6.456,P =0.011] and progress free survival [(PFS):(29 ± 5) months vs.(46 ± 5) months,x2 =6.742,P =0.009].Expression of HO-1 was up-regulated after HM13 gene was interfered (0.532±0.013 vs.0.395±0.011,t=13.93,P＜0.05).Conclusion The low expression of HM13 is associated closely with advanced stage and distant metastases of gastric carcinoma.
