Comparison of effects of dexmedetomidine and propofol on intestinal ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2015.07.017
- VernacularTitle:右美托咪定与异丙酚对大鼠肠缺血再灌注损伤影响的比较
- Author:
Changpeng DUAN
;
Yaying XIE
;
Jianshe YU
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Propofol;
Reperfusion injury;
Intestines
- From:
Chinese Journal of Anesthesiology
2015;35(7):837-839
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of dexmedetomidine and propofol on intestinal ischemia-reperfusion (I/R) injury in rats.Methods Forty male SPF Wistar rats, aged 2-3 months, weighing 185-230 g, were randomized into 4 groups (n=10 each) using a random number table: sham operation group (S group), intestinal I/R group (I/R group), propofol group (P group) and dexmedetomidine group (D group).Intestinal I/R was produced by occlusion of the superior mesenteric artery for 20 min followed by reperfusion.In P and D groups, propofol 10 mg/kg and dexmedetomidine 5 μg/kg were injected, respectively, via the femoral vein at 20 min before occlusion.At the end of 2 h reperfusion, the blood samples were collected from the inferior vena cava for determination of serum diamine oxidase (DAO) activity.A segment of the intestine of 5 cm in length was removed for microscopic examination with light microscope.The degree of damage to intestinal mucous membrane was scored according to Chiu.Results Compared to S group, the activity of DAO and Chiu' s score were significantly increased in I/R, P and D groups.Compared to I/R group, the activity of DAO and Chiu' s score were significantly decreased in P and D groups.Compared to P group, the activity of DAO and Chiu' s score were significantly decreased in group D.Conclusion Anesthetic dose of dexmedetomidine pretreatment reduces intestinal I/R injury in rats, and the effect is superior to that produced by propofol.
