Influence of propofol on endotoxin-induced expression of VEGF receptor 2 in renal glomerular endothelial cells of rats
10.3760/cma.j.issn.0254-1416.2015.04.030
- VernacularTitle:异丙酚对内毒素诱导大鼠肾小球血管内皮细胞VEGF受体2表达的影响
- Author:
Linlin ZHI
;
Zhiguo YUAN
- Publication Type:Journal Article
- Keywords:
Propofol;
Vascular endothelial growth factor receptor-2;
Endotoxemia;
Endothelial cells;
Kidney glomerulus
- From:
Chinese Journal of Anesthesiology
2015;35(4):499-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of propofol on endotoxin-induced expression of vascular endothelial growth factor (VEGF) receptor 2 in renal glomerular endothelial cells of rats.Methods The primarily cultured renal glomerular endothelial cells of Sprague-Dawley rats were seeded in 24-well plates at a density of 1×106 cells/ml (200 μl/well) and divided into 6 groups (n =35 each) using a random number table:control group (group C);introlipid group (group I);propofol group (group P);lipopolysaccharide (LPS) group (group L);LPS + introlipid group (group L+I);LPS + propofol group (group L+P).In group I,10％ introlipid was added with the final concentration of 4 μg/ml.In group P,propofol was added with the final concentration of 4 μg/ml.In group L,LPS was added with the final concentration of 10 μ g/ml.In group L+I,10％ introlipid was added with the final concentration of 4 μg/ml at 30 min before LPS with the final concentration of 10 μg/ml was added.In group L+P,propofol was added with the final concentration of 4 μg/ml at 30 min before LPS with the final concentration of 10 μg/ml was added.After 6 h of incutation,the cells were collected for measurement of cell permeability and VEGF receptor 2 mRNA expression (using RT-PCR),VEGF receptor 2 protein expression (by Western blot),and for examination of the morphology of cytoskeletal protein filamentous-actin (F-actin) with confocal microscope (by immunofluorescence).Results Compared with group C,the expression of VEGF receptor 2 mRNA and protein was significantly up-regulated,and the cell permeability was increased in L,L+I and L+P groups,and no significant changes were found in the parameters mentioned above in I and P groups.Compared with group L,the expression of VEGF receptor 2 mRNA and protein was significantly down-regulated,and the cell permeability was decreased in L+P group,and no significant changes were found in L+I group.F-actin connected closely between adjacent cells and a dense peripheral F-actin band was formed in C,I and P groups,while F-actin depolymerized,the peripheral F-actin band was disrupted,and cells shrank in L and L+I groups.In group L+P,a tighter intercellular connection of F-actin was observed and cytoskeleton was found to be intact.Conclusion Propofol can inhibit endotoxin-induced increase in the permeability of renal glomerular endothelial cells through down-regulating the expression of VEGF receptor 2 in rats.
