Role of endoplasmic reticulum stress in lung ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2015.04.009
- VernacularTitle:内质网应激在大鼠肺缺血再灌注损伤中的作用
- Author:
Jingjing XU
;
Zhiping WANG
- Publication Type:Journal Article
- Keywords:
Endoplasmic reticulum;
Stress;
Reperfusion injury;
Lung
- From:
Chinese Journal of Anesthesiology
2015;35(4):426-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of endoplasmic reticulum stress in lung ischemiareperfusion (I/R) injury in rats.Methods Fifty-six healthy male Sprague-Dawley rats,weighing 250-320 g,were randomly divided into 2 groups using a random number table:sham operation group (group S,n=16) and I/R group (n=40).Lung I/R was induced by clamping the left hilum of lung for 60 min followed by reperfusion.Before ischemia (T0) and at 0,1,2 and 4 h (T4) of reperfusion,PaO2 and PaCO2 were recorded.The rats were sacrificed,and lungs were removed for examination of pathological changes.Wet/dry lung weight ratio (W/D ratio) was calculated.The expression of molecular chaperones of 78-kDa glucose-regulated protein (GRP78) and C/EBP homologous protein (CHOP) protein and mRNA was detected in lung tissues using Western blot and real-time PCR,respectively.Results Compared with group S,PaO2was significantly decreased,PaCO2 and W/D ratio were increased,and the expression of GRP78 and CHOP protein and mRNA was up-regulated during reperfusion,and the pathological changes of lungs were found in group I/R.With the prolongation of reperfusion,the pathological changes of lungs were gradually aggravated,and the expression of GRP78 and CHOP protein and mRNA was gradually up-regulated in group I/R.Conclusion Excessive endoplasmic reticulum stress may be one of the pathophysiological mechanisms of lung I/R injury in rats.
