Role of calpain in sevoflurane anesthesia-induced apoptosis in hippocampal neurons of aged rats
10.3760/cma.j.issn.0254-1416.2015.04.008
- VernacularTitle:钙蛋白酶在七氟醚麻醉诱发老龄大鼠海马神经元凋亡中的作用
- Author:
Xiang LIU
;
Shuping HUO
;
Liang WANG
;
Jinhua HE
;
Tianbao YUAN
;
Xiuli WANG
;
Qiujun WANG
- Publication Type:Journal Article
- Keywords:
Calpain;
Anesthetics,inhalation;
Anesthesia;
Aged;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2015;35(4):423-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of calpain in sevoflurane anesthesia-induced apoptosis in hippocampal neurons of aged rats.Methods Fifty-four healthy female Sprague-Dawley rats,aged 18 months,weighing 450-550 g,were randomly divided into 3 groups (n=12 each) using a random number table:control group (group C),sevoflurane group (Sev group) and calpain inhibitor M DL28170 group (group M).In group C,the rats inhaled 50％ O2-50％N2 for 3 h.In Sev group,the rats inhaled 3％ sevoflurane for 3 h.In group M,MDL28170 10 mg/kg was injected via the tail vein,30 min later 3％ sevoflurane was inhaled for 3 h,and MDL28170 was simultaneously infused at 3.33 mg · kg 1 · h-1 via the tail vein.Nine rats in each group were selected,and cognitive function was assessed by using Morris water maze test at 30 min before anesthesia and 1-5 days after anesthesia.The escape latency and frequency of crossing the original platform were recorded.After the end of Morris water maze test performed at 30 min before anesthesia and 1-5 days after anesthesia,3 rats in each group were sacrificed,and hippocampal tissues were obtained for detection of cell apoptosis (by flow cytometry) and intracellular [Ca2+] i.Apoptotic rate was calculated.Results Compared with group C,the escape latency was significantly prolonged,and the frequency of crossing the original platform was decreased,and the apoptotic rate and intracellular [Ca2+]i were increased at 1 day after anesthesia in Sev and M groups.Compared with group Sev,the escape latency was significantly shortened,the frequency of crossing the original platform was increased,and the apoptotic rate was decreased at 1 day after anesthesia,and no significant change was found in intracellular [Ca2+]i in group M.Conclusion Calpain activation is involved in sevoflurane anesthesia-induced apoptosis in hippocampal neurons of aged rats.
