Differentially Expressed miRNAs in Peripheral Blood of Patients with Recurrent Colorectal Adenoma
10.3969/j.issn.1008-7125.2015.11.008
- VernacularTitle:结直肠腺瘤复发患者外周血miRNAs表达差异研究
- Author:
Qinghua LI
;
Zhiwen YANG
;
Mei WU
;
Ping XU
- Publication Type:Journal Article
- Keywords:
Colorectal Adenoma;
Recurrence;
MicroRNAs;
Differential Expression;
Microarray Analysis
- From:Chinese Journal of Gastroenterology
2015;(11):672-675
- CountryChina
- Language:Chinese
-
Abstract:
Background:MicroRNAs ( miRNAs ) is a class of small non-coding RNAs that regulate gene expression at posttranscriptional level. They are considered to play a critical role in the tumorigenesis and development of various tumors. Aims:To identify the recurrence-specific miRNAs in peripheral blood of patients with recurrent colorectal adenoma by miRNA array for providing sensitive and specific molecular markers for recurrence prediction. Methods:Fifty colorectal adenoma patients who had undergone polypectomy by colonoscopy were re-examined from Aug. 2012 to Sep. 2013. Four recurrent and four non-recurrent patients were selected and miRNA array was used to detect the expression profiles of miRNAs in peripheral blood samples. Differentially expressed miRNAs were identified between recurrent and non-recurrent patients. Real-time PCR was applied to verify the differentially expressed miRNAs identified by microarray. Results:Seven up-regulatedmiRNAs(hp hsa-miR-548ai,hsa-miR-4446-3p,hsa-miR-139-3p,hsa-miR-27a-5p,hsa-miR-10a,hsa-miR-23a and hsa-miR-486-3p)and 4 down-regulated miRNAs( hsa-miR-124,hsa-miR-3613-5p,hsa-miR-495 and hsa-miR-485-5p)were identified by microarray between recurrent and non-recurrent patients. Among them,seven miRNAs were validated by real-time PCR as consistent with microarray. Conclusions:Some differentially expressed miRNAs may be involved in the recurrence of colorectal adenoma,which gives clues for further investigation on recurrence predictors.
