Clinical application of three methods for the detection of brucellosis
10.3760/cma.j.issn.1008-6706.2015.22.008
- VernacularTitle:三种检测方法在人布鲁菌病检测中的应用
- Author:
Jing ZHANG
;
Yufei TANG
;
Lei WANG
;
Guifeng WANG
;
Biyong LIU
;
Jun LU
;
Xinhua LIU
- Publication Type:Journal Article
- Keywords:
Brucellosis;
Rose -bengal plate agglutination test;
Standard -tube agglutination test;
ELISA method;
Detection
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(22):3382-3384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect serum brucellosis in high risk population,three methods were used and their advantages and disadvantages was compared.Methods In accordance with the surveillance standard for brucello-sis(GB 16885 -1997)and brucellosis diagnostic criteria(WS269 -2007)in the prescribed method,rose -bengal plate agglutination test (RBPT),standard -tube agglutination test (SAT)and enzyme linked immunoassay assay (ELISA)were used to detect brucellosis and analysis of its diagnostic significance in the high risk population of sheep farm of Guoyang county in Anhui province.Results The positive rates of RBPT,SAT and ELISA were 19.1%,12.1% and 16.3% in 257 blood samples,respectively.Compared to SAT,the sensitivity,specificity,accura-cy and the area under the ROC curve of RBPT were 88.5%,91.8%,91.4%,0.81,respectively,which of ELISA were 93.9%,95.1%,94.9%,0.88.Conclusion The sensitivity,specificity,accuracy and the area under the ROC curve of ELISA were higher than those of other methods.Proper method,early surveillance and effective technology can help to control the occurrence and epidemic of brucellosis in the actual test work promotion.
