The application of MALDI-TOF mass spectrometry in pathogen detection from the nosocomial infection
10.3969/j.issn.1671-8348.2015.33.020
- VernacularTitle:MALDI-TOF质谱在院内感染致病菌检测中的应用
- Author:
Bowen TU
;
Weifeng SHI
;
Xiaodong HAN
;
Qiang DU
;
Xiaoyi XU
;
Chao HAO
- Publication Type:Journal Article
- Keywords:
cross infection MALDI-TOF mass spectrometry;
VITEK-2 biochemical analysis;
homology analysis
- From:
Chongqing Medicine
2015;(33):4665-4670
- CountryChina
- Language:Chinese
-
Abstract:
Objective Pathogens from the nosocomial infection have been analyzed by MALDI‐TOF microbial identification system ,to evaluate mass spectrometry analysis advantage and explore the mass spectrometry method .Methods The pathogens have been analyzed by MALDI‐TOF microbial identification system ,by compared with the VITEK‐2 compact detection in the tes‐ting time ,detection rate and the amounts of identified strains .The homology differences have been analyzed by comparison calcula‐tion of common peaks from the fingerprint spectrums .Results Thirty‐one Escherichia coli strains ,28 Klebsiella pneumonia strains and 9 unusual pathogen strains have been identified by MALDI‐TOF MS for only 1 hours .It has more advantages than VITEK‐2 in the testing time and other aspects .Conclusion Nosocomial infection of pathogen shows a point source propagation mode centering on the department .MALDI‐TOF mass spectrometry is able to rapidly and correctly identify the pathogen .MALDI‐TOF microbial i‐dentification system is expected to be the major detecting technique in the field of the pathogen monitor and resistance monitoring a ‐nalysis .
