The expression of hTERT and telomerase in human fetal gastric fibroblasts
10.3969/j.issn.1671-8348.2015.32.002
- VernacularTitle:人胎胃成纤维细胞hTERT和端粒酶的表达
- Author:
Yongbo CHENG
;
Liping GUO
;
Lei WANG
;
Dianchun FANG
- Publication Type:Journal Article
- Keywords:
fibroblast;
human telomerase reverse transcriptase;
telomerase
- From:
Chongqing Medicine
2015;(32):4469-4470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of human telomerase reverse transcriptase (hTERT ) and telomerase in hu‐man fetal gastric fibroblasts .Methods Human fetal gastric fibroblasts were isolated and cultured ,epithelial cells were exduded by CK‐18 immune staining .hTERT protein was determined by indirect immunofluorescence .Telomerase activity was analyzed using telomeric repeat amplification protocol assay (TRAP)while the same analysis in adult gastric fibroblast ,which as to be positive con‐trol .Results Cultured fibroblasts were CK‐18 negative .The positive hTERT immunostaining was detected in both cellular cyto‐plasm and nuclear compartments .Amplified telomeric repeats (about 170 bp) in human fetal gastric fibroblasts were longer than those (160 bp) in adult gastric fibroblasts .Conclusion hTERT and telomerase were expressed in human fetal gastric fibroblasts .
