Roles of GITR/GITRL signal system expression at bloodstream lymphocytes in children with asthma
10.3760/cma.j.issn.1008-6706.2015.23.012
- VernacularTitle:哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义
- Author:
Li FANG
;
Rongrong LI
;
Fangjun LUO
;
Liyan ZHAO
;
Peiying CHEN
;
Peifeng YU
- Publication Type:Journal Article
- Keywords:
Asthma;
T lymphocytes;
Receptor,tumor necrosis factor;
Signal transduction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(23):3558-3560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of blood CD +4 CD +25 Treg GITR,CD +4 T cell GITRL in children with asthma,and the role of them in asthmatic inflammation.Methods 50 cases of severe asthma were selected,and were controlled with thirty two healthy children.The venous blood was collected both in the period of acute episode and clinic remission.The mean fluorescence intensity of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL was detected by flow cytometry.Results The expression of CD +4 CD +25 Treg GITR in the asthma acute period group was (24.2 ±8.2)MFI,which was significantly lower than (28.5 ±6.0)MFI in the control group(t =2.5,P <0.05).While there was no significant difference of CD +4 T cell GITRL expression between the asthma acute period group[(5.2 ±3.6)MFI]and the control group[(4.6 ±1.0)MFI](t =1.1,P >0.05).Moreover,the expression of CD +4 CD +25 Treg GITR in the asthma in remission group after treatment was (29.5 ±8.3)MFI,which was significantly higher than that in acute period group before treatment(t =-9.9,P <0.01 ).Whereas,there was no significant difference of CD +4 T cell GITRL expression in asthma group between in remission after treatment[(5.7 ±3.6)MFI] and in acute period before treatment(t =-1.6,P >0.05).Furthermore,there was no significant correlation between levels of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL.Conclusion The level of CD +4 CD +25 Treg GITR in acute period asthmatic patients was decreased,but it was increased in remission,but no changes of CD +4 T cell GITRL expression were observed.GITR/GITRL signal system might be involved in the asthmatic inflammation procession.
