Role and expression level of 14-3-3 protein in lung tissues of asthmatic rats
10.3760/cma.j.issn.1008-6706.2015.23.013
- VernacularTitle:14-3-3蛋白在哮喘大鼠肺组织中的表达及意义
- Author:
Wenhui YAN
;
Zhenfeng JIANG
;
Xiasheng TONG
;
Jizhen ZHANG
;
Shujuan HE
;
Dongjiao LUO
- Publication Type:Journal Article
- Keywords:
14 -3 -3 protein;
Immunohistochemisty;
Asthma;
Rat
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(23):3561-3563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate possible role of 14 -3 -3 in the pathogenesis of asthma inflammation, the expression of 14 -3 -3 protein was observed in lung tissues of asthmatic rats.Methods Expressions of 14 -3 -3 protein was determined by immunohistochemisty method in lung tissues,and the relationship between 14 -3 -3 and asthma inflammation was analyzed.Results The location of positive expression of 14 -3 -3 protein was mainly at cytoplasm,while little at plasmalemma.The positive expression cell mostly was bronchial epithelial cell,others were lymphocytes,alveolar macrophages and vascular endothelial cells.On the other hand,the bronchial smooth muscle and vascular smooth muscle were negative expressed.Moreover,the expression level of 14 -3 -3 protein in asthma group [(0.353 ±0.023)absorbance]was significantly higher than that in the control group[(0.211 ±0.028 )absorbance] (t =10.969,P <0.01).Conclusion The results showed that the 14 -3 -3 protein was overexpressed in asthmatic lung tissue,it may play an important role in asthma inflammation through bronchial epithelial cells,lymphocytes, alveolar macrophages and vascular endothelial cell.
