The influence of all-trans retinoic acid on the craniomaxillofacial development of mice
10.3969/j.issn.1001-3733.2015.06.002
- VernacularTitle:全反式维甲酸对小鼠颅颌面部发育影响的研究
- Author:
Mu CHEN
;
Xu YANG
;
Xue LIU
;
Weicai WANG
;
Hongzhang HUANG
- Publication Type:Journal Article
- Keywords:
All-trans retinoic acid(atRA);
Mouse;
Craniomaxillofacial malformations
- From:
Journal of Practical Stomatology
2015;(6):748-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of all-trans retinoic acid (atRA)on craniomaxillofacial development of C57 mice. Methods:Pregnant C57BL mice were divided into 4 groups(n =5)at gestation day (GD)1 0.Mice in three atRA-induction groups were given atRA of 60,80 and 1 00 mg/kg,respectively.The mice in control group were given the equivalent volume of corn oil.All pregnant mice were sacrificed at GD1 9 and the embryos were collected.Stereo microscope was used to observe the craniomaxillofacial morphology.Standardized radiographs were taken and cephalometric analysis was performed.Results:The embryonic body length and body mass of control group surpassed those of 80 and 1 00 mg/kg atRA groups(P <0.05,P <0.01 ).atRA induced craniomaxillofacial malformations and maldevelopment.The mice induced by atRA exhibited a shorter mandibular body and more retrusive position of max-illary and mandibular(∠NAK and ∠NBD)when compared with their norm(P <0.01 ).Significant decrease in craniofacial length (Op-Rh)was observed in all atRA-induced groups(P <0.01 ).Decreases in cranial vault height(Fp-Os)and cranial vault length(Pa-Na)dimensions were observed in 80 and 1 00 mg/kg atRA groups(P <0.05,P <0.01 ).Conclusion:Exogenous atRA dose-depend-ently induces retardation of craniomaxillofacial morphology in embryo of C57BL mice by inhibition of the sagital and vertical dimension development of the bone.
