The expression of odontoblast relevant proteins in dental pulp stem cells induced by BMP-7

VernacularTitle:BMP-7诱导成牙本质细胞相关蛋白在 DPSCs 中的研究
Author: Juan MA ; Ruiqiao ZHU ; Xiaolei SHI ; Hui XU ; Yan JIN ; Lei JIN
Publication Type:Journal Article
Keywords: Dentin phosphprotein; Dentin matrix protein 1; Alkaline phosphatase; BMP-7; Dental pulp stem cells
From: Journal of Practical Stomatology 2015;(6):753-756
CountryChina
Language:Chinese
Abstract: Objective:To examine the expression of odontoblast related proteins in dental pulp stem cells(DPSCs)induced by BMP-7.Methods:DPSCs were cultured in the common culture medium or medium supplemented with 1 00 ng/ml BMP-7.Electron microscope,CCK8 and immunohistochemical staining were carried out to estimate the cell morphology and differentiation.Results:In-duced by BMP-7,the morphology of DPSCs was not changed,the proliferation of DPSCs was slower than that of the cells without BMP-7 treatment.DPSCs were negative for the expression of DSPP,DMP-7 and ALP.However,DPSCs were found strongly positive for DSPP,DMP-7 and ALP after the induction of BMP-7.Conclusion:BMP-7 induction may promote the differentiation of DPSCs.