Screening of differentialy expressed saliva proteins from oral lichen planus patients by two-dimensional fluo-rescence difference gel electrophoresis and mass spectrometry
10.3969/j.issn.1001-3733.2015.06.012
- VernacularTitle:应用双向荧光差异凝胶电泳和质谱技术筛选口腔扁平苔藓唾液差异蛋白
- Author:
Tiejun LIU
;
Kunshan LI
;
Jian LIU
;
Yongle QIU
;
Jingjing WU
;
Xin AN
;
Yanzhi XU
- Publication Type:Journal Article
- Keywords:
Oral lichen planus(OLP);
Saliva;
Proteomics;
2-D DIGE;
Liquid chromatography-mass spectrometry (LC-MS)
- From:
Journal of Practical Stomatology
2015;(6):793-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify differentially expressed saliva proteins of oral lichen planus(OLP)patients by two-dimensional fluo-rescence difference electrophoresis(2-D DIGE)and mass spectrometry(MS).Methods:3 pairs of saliva samples from OLP patients and matched healthy adults were collected.Saliva proteins were separated by 2-D DIGE and identified by liquid chromatography-mass spectrometry(LC-MS).Results:SDS-PAGE examination showed that the electrophoresis bands were clear and protein loss was rare. Protein dots were highly reproducible by 2-D DIGE.In average,the abundance of (31 7 ±71 )saliva protein spots were found in OLP pa-tients.4 highly reproducible spots were identified to be secretory IgA1 ,zincα-2-glycoprotein,salivary amylase and serum albumin by LC-MS and they were at higher level in OLP patients than those in the healthy controls.Conclusion:Secretory IgA1 ,zincα-2-glyco-protein,salivary amylase and serum albumin are highly expressed in the saliva of OLP patients,and may be related to the occurrence and development of oral lichen planus.
