Protective effect of trigonelline on rat cardiomyocytes during hypoxia and reoxygenation
10.3969/j.issn.1671-8348.2015.31.005
- VernacularTitle:胡芦巴碱对缺氧／复氧乳鼠心肌细胞的保护作用
- Author:
Kun HAN
;
Danping WANG
;
Lei YUAN
- Publication Type:Journal Article
- Keywords:
trigonelline;
myocytes,cardiac;
hypoxia/reoxygenation;
oxygen radicals;
mitochondria membrane;
membrane po-tential
- From:
Chongqing Medicine
2015;(31):4333-4335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of trigonelline in neonatal rat cardiomyocytes during hypoxia /reoxygenation and its mechanism .Methods The neonatal rat cardiomyocytes was randomly divided into the normal control group , the hypoxia/reoxygenation group and the trigonelline group .Flow cytometry was used to determine the apoptosis and mitochondrial membrane potential .The levels of SOD and MDA were measured in different groups .Western blot method was used to measure the procaspase‐9 ,cleaved caspase‐9 ,procaspase‐3 and cleaved caspase‐3 protein level .Results Trigonelline could inhibit apoptosis (P<0 .05) ,enhanced activity of SOD (P<0 .05) ,reduce production of MDA (P<0 .05) ,stabilize the mitochondrial membrane potential (P<0 .05) and activate caspase‐9 and caspase‐3 in neonatal rat cardiomyocytes during hypoxia/reoxygenation .Conclusion Trigo‐nelline could protect myocardial cells from injury caused by hypoxia and reoxygenation ,and the mechanism may be associated with anti‐lipid peroxidation and stabilizing mitochondria membrane potential .
