Effects of several harmful factors on expression of glycine receptor α1 subunit in neonatal rat myocardial cells
10.3969/j.issn.1000-4718.2015.11.013
- VernacularTitle:甘氨酸受体α1亚基在乳鼠心肌细胞的表达及损害性因素对其表达的影响
- Author:
Chutian CHEN
;
Daxiang LU
;
Renbin QI
;
Huadong WANG
- Publication Type:Journal Article
- Keywords:
Glycine receptor α1 subunit;
Lipopolysaccharides;
Hypoxia/reoxygenation;
Isoproterenol;
High concentration of glucose
- From:
Chinese Journal of Pathophysiology
2015;(11):2005-2008
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the expression of glycine receptorα1 subunit in neonatal rat myocardial cells and to investigate the effect of lipopolysaccharide (LPS), hypoxia/reoxygenation, isoproterenol (ISO) and high concentration of glucose (HG) on the expression of glycine receptorα1 subunit in the neonatal rat myocardial cells.METHODS:Neonatal rat myocardial cells were cultured in vitro.The expression of glycine receptorα1 subunit was detected by Western blotting. The neonatal rat myocardial cells were treated with LPS (20 mg/L), ISO (100 μmol/L) or high concentration of glucose (25 mmol/L) for 24 h, or were exposed to hypoxia for 3 h followed by reoxygenation for 3 h.Subsequently, the cell viabil-ity was measured by CCK-8 assay, and the expression of glycine receptorα1 subunit was determined by Western blotting. RESULTS:The expression of glycine receptor α1 subunit in the neonatal rat myocardial cells was positively detectable by Western blotting.Compared with control group, no significant difference of the cell viability ( P>0.05) in LPS group, ISO group, hypoxia/reoxygenation group and HG group was observed.The expression of glycine receptor α1 subunit was in-creased (P<0.01) in LPS group, ISO group and hypoxia/reoxygenatio group, but decreased (P<0.01) in HG group. CONCLUSION:Glycine receptorα1 subunit exists in the neonatal rat myocardial cells.A certain concentration of LPS or ISO, or hypoxia/reoxygenation for a certain period upregulate the expression of glycine receptorα1 subunit, but HG down-regulates the expression of glycine receptor α1 subunit in cultured neonatal rat myocardial cells.
