Preliminary inquiry EAE mouse atrophy of thymus relationship with disease se-veir ty
10.3969/j.issn.1000-484X.2015.11.003
- VernacularTitle:EAE小鼠胸腺萎缩与疾病严重程度关系的初步探究
- Author:
Gaohui WEI
;
Peiguo ZHENG
;
Junhua ZHANG
;
Xuehan MA
;
Xianchun MENG
;
Liang MING
;
Hongchun LIU
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune encephalomyelitis;
Thymus;
Atrophy
- From:
Chinese Journal of Immunology
2015;(11):1453-1456
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the relationship between atrophy of the thymus and disease severity in EAE.Methods:MOG35-55 peptide induced EAE in C57BL/6 mice and analyzed the relationship between the severity of EAE and thymic atrophy,Flow cytometry analysis was used to evaluate thymic CD4+CD8+DP cells,CD4+CD8-,CD4-CD8+SP cells in relation to the severity of the disease.Results:The number of thymocytes in mice with decreased tail tone was (20.25 ±3.49) ×106 ,hindlimb weakness(4.93 ± 0.85)×106,complete hindlimb paralysis(1.8 ±0.19) ×106,and forelimb and hindlimb paralysis(0.52 ±0.07) ×106,there were statistically significant differences between groups ( P<0.05 ).As the disease progresses, CD4+CD8+DP cells ratio decreased, CD4+CD8-,CD4-CD8+SP cell ratio increased,different disease groups was statistically significant difference (P<0.05).Conclusion: The atrophy of thymus was closely related to the severity of EAE.Migration of activated T cells in EAE may cause atrophy of thymus.
