Different spinal cord damage on apoptosis of rat secondary impact study
10.3969/j.issn.1000-484X.2015.11.005
- VernacularTitle:三种不同脊髓机械性损伤对大鼠继发性神经细胞凋亡的影响研究
- Author:
Min MAO
;
Ting ZHANG
;
Ying LIU
;
Guiqiong HE
;
Kejian WANG
- Publication Type:Journal Article
- Keywords:
Spinal cord contusion;
Persistent placeholder damage;
Secondary neural cell apoptosis
- From:
Chinese Journal of Immunology
2015;(11):1461-1464
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the spinal cord injury, spinal cord transection and persistent placeholder damage on the influence of secondary neural cell apoptosis in rats.Methods: Select 60 healthy male Wistar rats, numbered after using the random number table method is divided into A (18,spinal cord contusion),B (18,spinal cord transection),C (18,continuous placeholder),D (6,control),E (6,the control group only) groups of five,were observed at the 1,4,7 D after 5 group of rats nerve cell apoptosis index, spinal cord tissue Bcl-2,the expression of Bax,caspase 3 protein.Results:A,B,C three groups of rats after building 1 d are gray and white matter positive markers, and the gray matter and white matter of three groups of rats nerve cell apoptosis index differences statistically significant ( P<0.05);4 d,7 d after building gray matter and white matter of three groups of rats tend to place increased ap-optotic cells in the spinal cord index ( P<0.05);in building 1,4,7 d group C after rat spinal cord grey matter and white matter of apoptotic cell index was significantly higher than that of group A and group B, group B were significantly higher in group A and the differences were statistically significant (P<0.05).1,4,7 d after building A,B,C,D,E five group rats the Bcl-2,Bax,caspase-3 protein expression differences were statistically significant (P<0.05),1,4,7 d after building A,B,C the Bcl-2 of three groups of rats, Bax,caspase-3 protein expression was significantly higher than that of group D and group E ( P<0.05).Conclusion: Secondary rats after spinal cord injury of nerve cells apoptosis,apoptosis time,severity,and damage type and severity.
